| 1. | So qiaexpress was preferred in producing target proteins , and the purified products were to be used for further studies in activity analysis
)系统，故将qiaexpression系统大量表达的二片段进行了纯化和活性测定。 |
| 2. | Molecular weight of the purified product determined by high performance gel filter chromatography was 60634d , which was approximately equivalent to that of biostim ( 55036d )
结果必思添的保留时间为13 . 5分钟，分子质量55036d ；本文提取的kp荚膜糖蛋白保留时间为12 . 86分钟，分子质量为60634d 。 |
| 3. | 2 . pcr products can be purified by ctab method , which removing the dntp and primers . the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
2 、采用ctab进行pcr产物的纯化，以去除pcr产物中的引物和dntp ，所得产物可用于与t载体连接反应，以提高连接效率。 |
| 4. | Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot
将离心收集到的菌体超声破壁，离心分离，收集上清液，进行离子交换层析和分子筛分离纯化。 |
| 5. | The activity of the purified product was confirmed by indirect elisa analysis and was further confirmed by indirect immunofluorescence and immunohistochemistry after they were added to the culture medium of hepatocarcinoma cells
在大肠杆菌blzi中实现了scfv融合蛋白的表达。经ni nta柱纯化获得纯化产物，经间接elisa分析确定所得产物具有与hbsag结合的特异性。 |
| 6. | The large - scale expression product is concentrated and desalted by ultra - filter , and the target protein is separated by affinity chromatography with chitin , and then cut by cnbr . the purified product appears evident antibacterial activity with method of agar diffusion
采用优化的表达条件进行大量发酵，对上清液超滤浓缩脱盐后，采用几丁质基质进行亲和层析分离目的蛋白，溴化氰切割，琼脂孔穴法测抗菌活性。 |
| 7. | Maroclex is a range of low viscosity medicinal grade white oil . these oils are highly refined and purified products with food chemical stability , composed almost entirely of saturated hydrocarbons , unsaturated hydrocarbons which tend to react to form sludge , color bodies are removed in the refining process . medicinal grades are used in pharmaceutical , drug , cosmetic products and used as aids and lubricants for equipment and packaging that come into direct contact with food
优白为一种医药等级的高度精炼、极高纯度的矿物油产品，无色、无味、具备极佳的化学稳定性能，主要组成部分为饱和烃，容易引起反应而产生杂质以及颜色体的不饱和烃在精炼过程中除去，适用于直接与人体接触的医药、化妆品以及食品包装行业等。 |
| 8. | The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。 |
| 9. | The purified products were cloned into pgem - t - easy vector successfully , the cloned plasmids were transformed into e . coli . tgl . the specific recombinant plasmid was identified by molecular weight , pcr and restriction endonuclease analysis . the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert
经琼脂糖电泳检测，将大小与预计分子量一致的片段纯化后连接到pgem - t - easy克隆载体中，再转化大肠杆菌tgi感受态细胞，得到的转化子经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。 |
| 10. | At the same time an apparatus of zone melting is designed , the method of zone melting is used to purify the purified product of recrystalizing . after purification , the homogeneity and stability of the purified product of antioxidant d and bht are examined by high performance liquid chromatography ( hplc )
实验研究结果：防老剂d重结晶提纯工艺为：每次取10g工业品防老剂d ，经过碱洗后，去离子水洗涤至中性，溶于100ml乙醇中，同时用粉状活性炭( 1g次)脱色、过滤、母液静置2h后，再放入冰箱结晶2h 。 |
